Journal article
Investigation of the regulation of transcriptional changes in Ancylostoma caninum larvae following serum activation, with a focus on the insulin-like signalling pathway
BJD Datu, A Loukas, C Cantacessi, P O'Donoghue, RB Gasser
Veterinary Parasitology | ELSEVIER | Published : 2009
Abstract
The exit from dauer in the free-living nematode Caenorhabditis elegans is under the control of a single amphidial neuron (ASJ) of the insulin-like signalling pathway. Mutations of this pathway have the ability to suppress entry into the dauer stage. It has been postulated that insulin-like signalling plays a significant role in the response to serum stimulation in vitro of the third-stage larvae (L3s) of the canine hookworm Ancylostoma caninum. To test for the possible involvement of the insulin-like signalling cascade in the response to serum stimulation, the effects of two signalling stimulants (8-bromo cGMP and arecoline) and four inhibitors, namely 4,7-phenanthroline, phosphoinositide-3 ..
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Awarded by Australian Research Council
Funding Acknowledgements
We thank Anne Chester, Libbyjolly and Steven Kopp for their assistance in obtaining parasite material as well as Mai Tran, Najju Ranjit, Dean Jones, Luke Moertel, Angela Williamson, James Russell and P. Griffin for many helpful discussions. This research was Supported by the Australian Research Council (ARC) (DP0665230). A.L. is Supported by a Senior Research Fellowship from the National Health and Medical Research Council (NHMRC) of Australia. B.D. was Supported by an International Post Graduate Research Award and a University Of Queensland Graduate School Scholarship. B.D. also received travel grants from the ARC/NHMRC Research Network for Parasitology and the University Of Queensland (UQ GSRTA). The Australian Academy of Science and the Australian-American Fulbright Commission are also gratefully acknowledged (RBG).